SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Description 1. Cell counters for accurate and. Prime function 20 amol. 001 0. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. How to properly unlock iD series instrument. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. 7. Bioz Stars score: 98/100, based on 1 PubMed citations. The assay window spanned four orders of magnitude. 모든 제품. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. A: percent GFP-positive cells calculated using the classification feature in the software. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Plate chamber design of the SpectraMax category 1 microplate readers. Find regional support. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 305 reference level saturation. infoboxeu@moldev. Note: Additional readers with similar performance are listed. 5 decades, with sensitive detection of cells. To detect physical stability, Pdots were treated with water and incubated with plants. 1. More Product Information. SpectraMax ® iD3 and iD5 Multi-Mode . 0´ 104 8. 2 x 10-14. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. 303 unable to cal dark current. Promega. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Home > Search Results > Danaher Inc > spectramax i3x. com. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. All test measurements and calculations are handlediD5. Table 1. no. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The assay window spanned four orders of magnitude. Example: [Drawer]. Bioz Stars score: 98/100, based on 1 PubMed citations. ) wide and 65 cm (25. 0´ 104 6. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Figure 4. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. Molecular Devicesの全商品を見る. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. Home > Search Results > Danaher Inc > spectramax i3x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). 314 low lamp level warning. Contact Local Team. 305 reference level saturation. See Unpacking the SpectraMax Paradigm Instrument on page 1. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Fluorescence Microplate Reader Comparison Chart. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. Description 1. Read height should be optimized for . Each heating element is independently. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Prime function 20 amol. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Table 1. Italic and boldface type are used for emphasis. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. iD5. no. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Specifically, we present neurite. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Thetypeof luciferase(Renilla. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Download PDF. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 吸収度マイクロプレートリーダー SpectraMax® Mini. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 08, 0. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. i3x. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . 660-665 Eskdale Road. Then, the cell area was calculated for each. Reporter Assay. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. The corrected absorbance is within 5% of the value. Also refer to this Multimode Reader Comparison Chart. The SpectraMax i2X is used for cell migration, cell. , 2014; Xiong et al. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Explore a wealth of applications with the future-ready SpectraMax i3x. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax ® i3x reader provides the. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Quickly and easily expand the capabilities of your SpectraMax Paradigm or i3x Multi-Mode Microplate reader to include Tunable Wavelength detection using the. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 2 only) SpectraMax M-series Microplate readers. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. To learn more about the device, please watch this short video. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. StainFree Cell Detection Algorithm. Description 1. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Detection of pro-inflammatory factors. 2 only) SpectraMax M-series Microplate readers. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Examples: “Press carefully to engage,” “ Do not. D 1 1. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. SpectraMax Microplate Readers. 312 gain calibration failed. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. ATP (“flash” luminescence using . Generate more data points without the need to dilute. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. SpectraMax i3x. Patented user-exchangeable cartridge design expands the system's detection capability making it. 6% for the on-board optics (monochromators). applications, including dual luciferase and ATP assays. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. 8 Antibodies and. Multimode Reader Comparison Chart. HTRF IP standard curve. Thetypeof. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The SoftMax Pro Software provides integrated instrument control. 3. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. 314 low lamp level warning. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. 313 reference gain check fail. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Enzyme Activation Analysis;. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. . 22 μ m fi ltration unit (Sigma, cat. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. Louis, MO) and used as received without further purification. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Sensitive TRF detection of two simple, mix-and-measure assay workflows. *On the SpectraMax i3x reader only. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. The drawing shows a standard microplate of 15 mm height covered with a lid. See SpectraMax® Microplate Reader User Manual Download Page. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Patented user-exchangeable cartridge design expands the system's detection capability making it. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. 6 in. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. i3. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. Robust,. Next,. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. 58% for the detection module and 2. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 0. The standard solutions (0. Time-. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. Materials and methodsWe would like to show you a description here but the site won’t allow us. The treatments were. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. The SpectraMax i2X is used for cell migration, cell. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Introduction. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Multimode Readers Comparison Chart. GFP expression imaged on the cytometer. Manufacturers try to design their instruments so they can be. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Supplier Page. Analysis. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 00800 665 32860. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Multimode Reader Comparison Chart. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax i3x Multi-Mode Detection Platform User Guide. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SpectraMax i3x Multi-Mode Detection Platform User Guide. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. SeeSpectraMax MiniMaxImagingCytometeronpage85. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. applications, including dual luciferase and ATP assays. Prepare your iD series instrument for shipment. Features. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. It features a modular design that allows users to add or change detection cartridges according to their needs. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Figure 4. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). The information below applies to a standard 96 well plate, set to a linear shake mode. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 2. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. Methods. 313 reference gain check fail. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Description 1. These concentrations are then used to perform western blots. TUNE Tunable Wavelength Detection Cartridge. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. each new assay. Figure 4. With optimized reagents, option to operate the readers. Figure 1. Price from $9. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The size and fluorescence intensity were tested for 48 h. In addition, Image J was used to quantify the. SpectraMax® iD3/iD5. 304 signal level saturation. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. Features. SpectraMax Quant dsDNA Assay Kits. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The CellTiter-Glo assay from Promega uses luciferase. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. The chamber. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Fluorescence Microplate Reader Comparison Chart. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. , Fisher Scienti fi c, cat. See SpectraMax® Microplate Reader User Manual Download Page. Microplate optimization should be performed the first time the assay is run, and subsequently for . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. This is not due to a temperature-dependent alteration in cellular autofluorescence,. This way you’ll save time on finding the necessary info. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. o For example: Ex: 485/Em 525/Cutoff 515 4. Category 2 and 3 microplate readers use gradient heating as well. 02, 0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Molecular Devices are known for both the reliability and the wide uses of their products. 0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Introduction. , 2021). Molecular Devices Corporation. Increased fluorescence performance. User-upgradeable detection modules allow you to expand the capabilities of your microp. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Recommended settings for SpectraMax iD5 and i3x readers. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Molecular Devices Software. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. Microplate Reader. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. QBT Fatty Acid Transporter Assay. , 2021). SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. User-upgradeable detection modules allow you to expand the capabilities. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. i3. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. ATP standard curves read on SpectraMax microplate readers. In this product video, see how the SpectraMax M5 quickly reads samples. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. See SpectraMax® Microplate Reader User Manual Download Page. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Prepare your i3/i3x instrument for shipment. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. ) wide and 65 cm (25. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. each new lot of plates, to ensure that the center of each well is read. The dynamic range of the assay is about 3. The MPO assay was conducted according to the. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Multimode Reader Comparison Chart. pathways and protein activation and expression in one system. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Published Dec 16, 2020 | Updated Dec 16, 2020. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Average 98 stars, based on 1 article reviews. 99 to $1999. Spectramax I3x, supplied by Danaher Inc, used in various techniques. AquaMax Microplate Washer. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. ) deep, preferably with access on all sides. 306 plate air cal fail,low light. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only.